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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases

Anti-NEK4 antibody (ab95176)

Anti-NEK4 antibody (ab95176)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to NEK4
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-NEK4 antibody
    See all NEK4 primary antibodies
  • Description

    Rabbit polyclonal to NEK4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 475-525 of human NEK4 (NP_003148.2)

  • Positive control

    • HeLa cell lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab95176 was affinity purified using an epitope specific to NEK4 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK4 antibody (ab95176)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK4 antibody (ab95176)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NEK4 with ab95176 at 1/200 (1µg/ml). Detection: DAB.
  • Western blot - Anti-NEK4 antibody (ab95176)
    Western blot - Anti-NEK4 antibody (ab95176)
    All lanes : Anti-NEK4 antibody (ab95176) at 0.04 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg/ml
    Lane 2 : HeLa cell lysate at 15 µg/ml
    Lane 3 : HeLa cell lysate at 5 µg/ml

    Predicted band size: 95 kDa



    Detection: Chemiluminescence with exposure times of 3 minutes.
  • Immunoprecipitation - Anti-NEK4 antibody (ab95176)
    Immunoprecipitation - Anti-NEK4 antibody (ab95176)
    Immunoprecipitation of NEK4 from HeLa cell lysate (1mg) using ab95176 at 3µg/mg lysate , followed by Western blotting of 20% of the immunoprecipitate: (1) ab95176 at 1 µg/ml, (2) control IgG
    Detection: Chemiluminescence with exposure times of 30 seconds.

    All lanes : Anti-NEK4 antibody (ab95176) at 0.04 µg/ml

    Lane 1 : IP: Anti-NEK4
    Lane 2 : IP: ab95176
    Lane 3 : IP: control IgG

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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