Anti-NEK4 antibody (ab95176)
Key features and details
- Rabbit polyclonal to NEK4
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NEK4 antibody
See all NEK4 primary antibodies -
Description
Rabbit polyclonal to NEK4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey, Gorilla, Orangutan -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 475-525 of human NEK4 (NP_003148.2)
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Positive control
- HeLa cell lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab95176 was affinity purified using an epitope specific to NEK4 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NEK4 with ab95176 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-NEK4 antibody (ab95176) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg/ml
Lane 2 : HeLa cell lysate at 15 µg/ml
Lane 3 : HeLa cell lysate at 5 µg/ml
Predicted band size: 95 kDa
Detection: Chemiluminescence with exposure times of 3 minutes. -
Immunoprecipitation of NEK4 from HeLa cell lysate (1mg) using ab95176 at 3µg/mg lysate , followed by Western blotting of 20% of the immunoprecipitate: (1) ab95176 at 1 µg/ml, (2) control IgG
Detection: Chemiluminescence with exposure times of 30 seconds.
All lanes : Anti-NEK4 antibody (ab95176) at 0.04 µg/ml
Lane 1 : IP: Anti-NEK4
Lane 2 : IP: ab95176
Lane 3 : IP: control IgG