Anti-Raf1 (phospho S296) antibody (ab60985)
Key features and details
- Rabbit polyclonal to Raf1 (phospho S296)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Raf1 (phospho S296) antibody
See all Raf1 primary antibodies -
Description
Rabbit polyclonal to Raf1 (phospho S296) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of Raf1 only when phosphorylated at serine 296. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from human Raf1 around the phosphorylation site of serine 296 (S-S-SP-P-N).
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Positive control
- Human breast carcinoma tissue, extracts from 293 cells treated with PMA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raf1 (phospho S296) antibody (ab60985)Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab60985 at 1/50-1/100 dilution, with and without the immunising peptide.
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Western blot - Anti-Raf1 (phospho S296) antibody (ab60985)All lanes : Anti-Raf1 (phospho S296) antibody (ab60985) at 1/500 dilution
Lane 1 : Extracts from 293 cells treated
with PMA (125ng/ml, 30mins).
Lane 2 : Extracts from 293 cells treated
with PMA (125ng/ml, 30mins), and the immunising phosphopeptide.
Predicted band size: 73 kDa
Observed band size: 73 kDa
