Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3433(2)] to Raf1 (phospho S259)
  • Suitable for: WB, Dot blot
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Raf1 (phospho S259) antibody [EPR3433(2)]
    See all Raf1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3433(2)] to Raf1 (phospho S259)

  • Host species

    Rabbit

  • Specificity

    This antibody only detects Raf1 when phosphorylated at Serine 259.

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Raf1 aa 250-350 (phospho S259) (Cysteine residue). The exact sequence is proprietary.
    Database link: P04049

  • Positive control

    • WB: Treated C6, NIH/3T3 and HEK-293 whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)
    Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)
    All lanes : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/5000 dilution (Purified)

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) treated with 100nM Phorbol-12-myristate-13-acetate for 1 hour whole cell lysates
    Lane 3 : HEK-293 (Human embryonic kidney epithelial cell) treated with 100nM Phorbol-12-myristate-13-acetate for 1 hour whole cell lysates, then incubated with phosphatase

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 73 kDa
    Observed band size: 73 kDa

  • Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)
    Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)
    All lanes : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/1000 dilution (Purified)

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with 5uM forskolin for 10 minutes whole cell lysates
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) treated with 5uM forskolin for 10 minutes whole cell lysates, then incubated with phosphatase

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 73 kDa
    Observed band size: 73 kDa

  • Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)
    Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)
    Lane 1 : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/1000 dilution (Purified)
    Lanes 2-3 : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 2 : C6 (Rat glial tumor glial cell) treated with 30uM forskolin for 20 minutes whole cell lysates
    Lane 3 : C6 (Rat glial tumor glial cell) treated with 30uM forskolin for 20 minutes whole cell lysates, then incubated with phosphatase

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 73 kDa
    Observed band size: 73 kDa

  • Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)
    Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)
    All lanes : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/5000 dilution

    Lane 1 : HEK293 whole cell lysate
    Lane 2 : HEK293 whole cell lysate treated with 100nM Phorbol-12-myristate-13-acetate for 1 hour
    Lane 3 : HEK293 whole cell lysate treated with 100nM Phorbol-12-myristate-13-acetate for 1 hour then incubated with phosphatase

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 73 kDa
    Observed band size: 73 kDa


    Exposure time: 1 second


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)
    Dot Blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)

    Dot blot analysis of Raf1 (pS259) peptide (Lane 1) and Raf1 non-phospho peptide (Lane 2) labelling Raf1 (pS259) with ab173539 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)
    Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)
    All lanes : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/1000 dilution

    Lane 1 : 293T cell lysates
    Lane 2 : 293T cell lysates treated with Okadaic Acid + Calyculin A

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 73 kDa

  • Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)
    Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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