Anti-Raf1 (phospho S642) antibody (ab192664)
Key features and details
- Rabbit polyclonal to Raf1 (phospho S642)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Raf1 (phospho S642) antibody
See all Raf1 primary antibodies -
Description
Rabbit polyclonal to Raf1 (phospho S642) -
Host species
Rabbit -
Specificity
ab192664 detects endogenous levels of Raf1 only when phosphorylated at serine 642. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human Raf1 (phospho S642) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. (T-T-S(p)-P-R) .
Database link: P04049 -
Positive control
- Extracts from HuvEc cells and K562 cells treated with PMA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab192664 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Raf1 (phospho S642) antibody (ab192664)All lanes : Anti-Raf1 (phospho S642) antibody (ab192664) at 1/500 dilution
Lane 1 : Extracts from HuvEc cells treated with PMA
Lane 2 : Extracts from K562 cells treated with PMA
Lane 3 : Extracts from K562 cells treated with PMA with antigen-specific peptide
Predicted band size: 73 kDa
