Anti-Raf1 (phospho S43) antibody [EPR5692(2)] (ab150365)
![Anti-Raf1 (phospho S43) antibody [EPR5692(2)] (ab150365)](https://www.abcam.com/ps/products/150/ab150365/Images/ab150365-156936-anti-raf1-phospho-s43-antibody-epr56922-western-blot.jpg "Anti-Raf1 (phospho S43) antibody [EPR5692(2)] (ab150365)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5692(2)] to Raf1 (phospho S43)
- Suitable for: WB
- Reacts with: Human
Overview
-
Product name
Anti-Raf1 (phospho S43) antibody [EPR5692(2)]
See all Raf1 primary antibodies -
Description
Rabbit monoclonal [EPR5692(2)] to Raf1 (phospho S43) -
Host species
Rabbit -
Specificity
ab150365 only detects Raf1 when phosphorylated at serine 43. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
A phospho specific peptide corresponding to residues surrounding serine 43 of Human Raf1.
-
Positive control
- HeLa cell lysates treated with Lambda Phosphatase.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5692(2) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Raf1 (phospho S43) antibody [EPR5692(2)] (ab150365)All lanes : Anti-Raf1 (phospho S43) antibody [EPR5692(2)] (ab150365) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : HeLa cell lysates treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 73 kDa
-
![Anti-Raf1 (phospho S43) antibody [EPR5692(2)] (ab150365)](https://www.abcam.com/ps/products/150/ab150365/Images/ab150365-2-benefits-of-recombinant-antibodies.png)
Anti-Raf1 (phospho S43) antibody [EPR5692(2)] (ab150365)
