Anti-Raf1 antibody (ab230850)
Key features and details
- Rabbit polyclonal to Raf1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Raf1 antibody
See all Raf1 primary antibodies -
Description
Rabbit polyclonal to Raf1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Orangutan
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Immunogen
Synthetic peptide corresponding to Mouse Raf1 aa 50-150 (N terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q99N57 -
Positive control
- WB: Neuro-2a and Jurkat whole cell lysate (ab7899). IHC-P: Human skin tissue. Flow Cyt (Intra): Neuro-2a cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Raf1 antibody (ab230850)Anti-Raf1 antibody (ab230850) at 1/1000 dilution + Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 73 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raf1 antibody (ab230850)Formalin-fixed, paraffin-embedded human skin tissue stained for Raf1 using ab230850 at 1/100 dilution in immunohistochemical analysis.
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Western blot - Anti-Raf1 antibody (ab230850)Anti-Raf1 antibody (ab230850) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 73 kDa
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Flow Cytometry - Anti-Raf1 antibody (ab230850)Flow cytometric analysis of Neuro-2a (mouse neuroblastoma cell line) cell line (right histogram) labeling Raf1 with ab230850 at 1/10 dilution compared with a negative control cell line (left histogram). FITC-conjugated donkey anti-rabbit secondary antibodies were used for the analysis.
