Anti-Aldolase C antibody - Astrocyte Marker (ab87122)
Key features and details
- Rabbit polyclonal to Aldolase C - Astrocyte Marker
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Aldolase C antibody - Astrocyte Marker
See all Aldolase C primary antibodies -
Description
Rabbit polyclonal to Aldolase C - Astrocyte Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Chimpanzee, Monkey
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Immunogen
Synthetic peptide corresponding to Mouse Aldolase C aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101304) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab87122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 39 kDa). ICC Use a concentration of 5 µg/ml. Target
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Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. -
Sequence similarities
Belongs to the class I fructose-bisphosphate aldolase family. - Information by UniProt
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Database links
- Entrez Gene: 493951 Chimpanzee
- Entrez Gene: 504584 Cow
- Entrez Gene: 230 Human
- Entrez Gene: 11676 Mouse
- Entrez Gene: 24191 Rat
- Omim: 103870 Human
- SwissProt: Q5R1X4 Chimpanzee
- SwissProt: P09972 Human
see all -
Alternative names
- ALDC antibody
- ALDO C antibody
- aldoc antibody
see all
Images
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Immunocytochemistry - Anti-Aldolase C antibody - Astrocyte Marker (ab87122)
ab87122 staining Fructose-bisphosphate aldolase C in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab87122 at 5µg/ml and ab4674, Chicken polyclonal to GFAP. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150176, Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-Aldolase C antibody - Astrocyte Marker (ab87122)All lanes : Anti-Aldolase C antibody - Astrocyte Marker (ab87122) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
The 44 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Mouse Fructose-bisphosphate aldolase C.
Protocols
Datasheets and documents
References (3)
ab87122 has been referenced in 3 publications.
- Cardona C et al. Identification of the iduronate-2-sulfatase proteome in wild-type mouse brain. Heliyon 5:e01667 (2019). PubMed: 31193135
- Xiao ZD et al. Energy stress-induced lncRNA FILNC1 represses c-Myc-mediated energy metabolism and inhibits renal tumor development. Nat Commun 8:783 (2017). PubMed: 28978906
- Li J et al. EZH2-mediated H3K27 trimethylation mediates neurodegeneration in ataxia-telangiectasia. Nat Neurosci 16:1745-53 (2013). PubMed: 24162653
Images
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Immunocytochemistry - Anti-Aldolase C antibody - Astrocyte Marker (ab87122)
ab87122 staining Fructose-bisphosphate aldolase C in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab87122 at 5µg/ml and ab4674, Chicken polyclonal to GFAP. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150176, Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-Aldolase C antibody - Astrocyte Marker (ab87122)All lanes : Anti-Aldolase C antibody - Astrocyte Marker (ab87122) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
The 44 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Mouse Fructose-bisphosphate aldolase C.
