Anti-Raf1 (phospho S338) antibody (ab51042)
Key features and details
- Rabbit polyclonal to Raf1 (phospho S338)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Raf1 (phospho S338) antibody
See all Raf1 primary antibodies -
Description
Rabbit polyclonal to Raf1 (phospho S338) -
Host species
Rabbit -
Specificity
Detects endogenous levels of Raf1 only when phosphorylated at serine 338. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide:
RDSSY
, corresponding to amino acids 336-340 of Human Raf1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Raf1 (phospho S338) antibody (ab51042)All lanes : Anti-Raf1 (phospho S338) antibody (ab51042) at 1/500 dilution
Lane 1 : Extract of NIH3T3 cells treated with PMA (125ng/ml, 15 min) and blocked with phospho peptide.
Lane 2 : Extract of NIH3T3 cells treated with PMA (125ng/ml, 15 min)
Predicted band size: 73 kDa
Observed band size: 73 kDa
