Anti-Raf1 (phospho Y341) antibody (ab59223)
Key features and details
- Rabbit polyclonal to Raf1 (phospho Y341)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Raf1 (phospho Y341) antibody
See all Raf1 primary antibodies -
Description
Rabbit polyclonal to Raf1 (phospho Y341) -
Host species
Rabbit -
Specificity
ab59223 detects endogenous levels of Raf1 only when phosphorylated at tyrosine 341. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from human Raf1 around the phosphorylation site of tyrosine 341 (S-Y-YP-W-E).
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Positive control
- Human pancreas tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab59223 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Raf1 (phospho Y341) antibody (ab59223)All lanes : Anti-Raf1 (phospho Y341) antibody (ab59223) at 1/500 dilution
Lane 1 : Jurkat cell extracts treated with paclitaxel
Lane 2 : Jurkat cell extracts treated with paclitaxel with antigen-specific peptide
Predicted band size: 73 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raf1 (phospho Y341) antibody (ab59223)ab59223 (1/50 dilution) staining Raf1 in human pancreas tissue by Immunohistochemistry (Paraffin section); in the absence or presence of the immunising peptide.
