Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Timeless antibody [EPR5275] (ab109512)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5275] to Timeless
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Human

Product name

Anti-Timeless antibody [EPR5275]
See all Timeless primary antibodies
Description

Rabbit monoclonal [EPR5275] to Timeless
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, Jurkat, JAR, and BxPC-3 cell lysates, Human colon and Human tonsil tissues
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5275
Isotype

IgG
Research areas

Western blot - Anti-Timeless antibody [EPR5275] (ab109512)

All lanes : Anti-Timeless antibody [EPR5275] (ab109512) at 1/50000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : JAR cell lysate
Lane 4 : BxPC-3 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 139 kDa
Observed band size: 150 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-Timeless antibody [EPR5275] (ab109512)

Immunocytochemistry/Immunofluorescence analysis of HT-29 (Human colorectal adenocarcinoma cell line) labeling Timeless with Purified ab109512 at 1/1000 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Timeless antibody [EPR5275] (ab109512)

Immunohistochemical analysis of paraffin-embedded Human colon tissue using ab109512 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Timeless antibody [EPR5275] (ab109512)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109512 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Timeless antibody [EPR5275] (ab109512)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com