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Human IL-2 ELISA Kit, Fluorescent (ab229441)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.7 pg/ml
  • Range: 3.9 pg/ml - 16000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Fluorescent
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IL-2 ELISA Kit, Fluorescent
    See all IL-2 kits

  • Detection method

    Fluorescent

  • Precision

    Inter-assay
    Sample n Mean SD CV%
    IL-2 protein 24 6%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    3.7 pg/ml

  • Range

    3.9 pg/ml - 16000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 95 93% - 97%
    Hep Plasma 91 82% - 95%
    EDTA Plasma 100 95% - 105%
    Cit plasma 83 77% - 98%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    IL-2 (Interleukin-2) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 (Interleukin-2) protein in human cell culture supernatant, plasma and serum samples.


    This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
    If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.


    The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material.  CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

  • Notes

    IL-2, also known as T cell growth factor (TCGF), is a glycosylated alpha-helical polypeptide, synthesized as a 153 amino acid (aa) precursor with a 20 aa signal peptide and a 133 aa mature chain. It is secreted by activated CD4+ and CD8+ T cells, neurons, microglia and hematopoietic stem cells in response to antigenic or mitogenic stimulation.  IL-2 is required for T-cell proliferation, Natural Killer cells (NK) cytolytic activity, differentiation of regulatory T cells, modulation of T helper (Th) cell differentiation and activation-induced cell death.  In particular, IL-2 modulates the expression of receptors for other cytokines and transcription factors, therefore regulating cytokine cascades that correlate with each of the Th differentiation states.

    Complete deficiency of IL-2 has been implicated in severe combined immunodeficiency, whereas reduction of the IL-2 correlates with reduced function of CD4+CD25+ regulatory T cells and destabilization of immune homeostasis leading to autoimmune disease. Increased expression of IL-2 has also been implicated in inflammatory conditions such as inflammatory bowel disease and chronic liver diseases. IL-2 therefore is both a immune stimulator and immune suppressor cytokine which efficiently controls the immune system to deal with autoimmunity and adaptive immune response.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Stoplight Red Substrate 1 x 120µl
    10X Human IL-2 Capture Antibody 1 x 600µl
    10X Human IL-2 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl
    Antibody Diluent 4BI 1 x 6ml
    Human IL-2 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated Black 96-Well Microplate 1 unit
    Stoplight Red Substrate Buffer 1 x 12ml

  • Research areas

  • Function

    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

  • Involvement in disease

    Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.

  • Sequence similarities

    Belongs to the IL-2 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • OTTHUMP00000164090
    • POIL2
    • T Cell Growth Factor
    • T-cell growth factor
    • TCGF
    see all

  • Database links

    Images

    • Other - Human IL-2 ELISA Kit, Fluorescent (ab229441)
      Other - Human IL-2 ELISA Kit, Fluorescent (ab229441)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human IL-2 (Interleukin-2) standard curve in Sample Diluent NS
      Example of human IL-2 (Interleukin-2) standard curve in Sample Diluent NS

      The IL-2 (Interleukin-2) standard curve was prepared as described in Section 10.

    • Specificity of IL-2 signal on stimulated and non stimulated media supernatants
      Specificity of IL-2 signal on stimulated and non stimulated media supernatants

      Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 µg/mL streptomycin. Cells were cultured for 2 days at 37⁰C in the presence or absence of PHA. The concentrations of IL-2 were interpolated from the calibration curve and corrected for sample dilution. The mean IL-2 concentration was determined to be 16 pg/mL in unstimulated PBMC supernatants and 11,460 pg/mL in stimulated PBMC supernatants

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