Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20863-3] to WSX-1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free
    See all WSX-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20863-3] to WSX-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type mouse spleen and bone marrow tissue lysates.

  • General notes

    ab273155 is the carrier-free version of ab220359. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)
    Western blot - Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)
    All lanes : Anti-WSX-1 antibody [EPR20863-3] (ab220359) at 1/1000 dilution

    Lane 1 : Wild-type mouse spleen (1) tissue lysate
    Lane 2 : Wild-type mouse spleen (2) tissue lysate
    Lane 3 : Wild-type mouse spleen (3) tissue lysate
    Lane 4 : IL27Ra knockout mouse spleen (1) tissue lysate
    Lane 5 : IL27Ra knockout mouse spleen (2) tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 69 kDa
    Observed band size: 110/120 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Data from collaborator.   

    Exposure time: 1 minute.

    WSX-1 = IL27Ra

    These data were developed using the same antibody clone in a different buffer formulation containing PBS, glycyerol, BSA and sodium azide (ab220359).

  • Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)
    Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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