Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)
![Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)](https://www.abcam.com/ps/products/273/ab273155/Images/ab273155-392338-antiwsx1antibodyepr208633westernblotwildtypemousespleen.jpg "Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20863-3] to WSX-1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free
See all WSX-1 primary antibodies -
Description
Rabbit monoclonal [EPR20863-3] to WSX-1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type mouse spleen and bone marrow tissue lysates.
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General notes
ab273155 is the carrier-free version of ab220359. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20863-3 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)All lanes : Anti-WSX-1 antibody [EPR20863-3] (ab220359) at 1/1000 dilution
Lane 1 : Wild-type mouse spleen (1) tissue lysate
Lane 2 : Wild-type mouse spleen (2) tissue lysate
Lane 3 : Wild-type mouse spleen (3) tissue lysate
Lane 4 : IL27Ra knockout mouse spleen (1) tissue lysate
Lane 5 : IL27Ra knockout mouse spleen (2) tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 110/120 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Data from collaborator.
Exposure time: 1 minute.
WSX-1 = IL27Ra
These data were developed using the same antibody clone in a different buffer formulation containing PBS, glycyerol, BSA and sodium azide (ab220359).
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![Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)](https://www.abcam.com/ps/products/273/ab273155/Images/ab273155-2-benefits-of-recombinant-antibodies.png)
Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)
