Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17924] to c-Myc (phospho S62) - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, IP, Dot blot, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free
    See all c-Myc primary antibodies

  • Description

    Rabbit monoclonal [EPR17924] to c-Myc (phospho S62) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Dot
    Human
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, NIH/3T3 and C6 whole cell lysates. IHC-P: Human endometrium cancer, mouse spleen and rat testis tissues. ICC/IF: HeLa cells. IP: HeLa whole cell lysate treated with 200nM TPA for 10 minutes. Flow Cyt: HeLa cells.

  • General notes

    Ab232691 is the carrier-free version of ab185656. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232691 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Flow Cytometry - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
    Flow Cytometry - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling c-Myc (phospho S62) with purified ab185656 at 1/150 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185656).

  • Dot Blot - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
    Dot Blot - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)

    Dot blot analysis of c -Myc (phospho T58) peptide (Lane 1), c-Myc non-phospho peptide (a control peptide for c-Myc phospho T58) (Lane 2), c-Myc (phospho S62) peptide (Lane 3), and c-Myc non-phospho peptide (a control peptide for c-Myc phospho S62) (Lane 4), labeled using ab185656 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Lanes 1, 2 and 4 are control peptides, lane 3 contains the immunogen peptide.

    Exposure time=3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185656).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling c-Myc (phospho S62) with ab185656 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Nuclear and cytoplasmic staining on mouse spleen is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185656).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling c-Myc (phospho S62) with ab185656 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Nuclear staining on rat testis is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185656).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
    Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling c-Myc (phospho S62) with ab185656 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing nuclear staining on HeLa cells.
    The staining decreased after blocking with phospho peptide (100μg/ml) overnight.

    The control peptide is a non-phospho peptide.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab185656 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185656).

  • Immunoprecipitation - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
    Immunoprecipitation - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)

    c-Myc (phospho S62) was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 200nM TPA for 10 minutes with ab185656 at 1/50 dilution.

    Western blot was performed from the immunoprecipitate using ab185656 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

    Lane 1: HeLa whole cell lysate treated with 200nM TPA for 10 minutes,10 µg (Input).

    Lane 2: ab185656 IP in HeLa whole cell lysate treated with 200nM TPA for 10 minutes.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185656 in HeLa whole cell lysate treated with 200nM TPA for 10 minutes.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185656).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)

    This IHC data was generated using the same anti-phospho S62 c-Myc  antibody clone, EPR17924, in a different buffer formulation (cat# ab185656).

    Immunohistochemical analysis of paraffin-embedded Human endometrium cancer tissue labeling c-Myc (phospho S62) with ab185656 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Nuclear staining on cancer cells of Human endometrial cancer is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
    Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)

    This ICC/IF data was generated using the same anti-phospho S62 c-Myc  antibody clone, EPR17924, in a different buffer formulation (cat# ab185656).

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling c-Myc (phospho S62) with ab185656 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing nuclear staining on HeLa cells.
    The staining decreased after blocking with phospho peptide (100μg/ml) overnight.

    The control peptide is a non-phospho peptide.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab185656 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
    Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)

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