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Mouse CD27 ELISA Kit (ab233619)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 10.9 pg/ml
  • Range: 31.3 pg/ml - 2000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse CD27 ELISA Kit
    See all CD27 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 5.3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 2%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    10.9 pg/ml

  • Range

    31.3 pg/ml - 2000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 104 98% - 108%
    Serum 100 93% - 106%
    Hep Plasma 105 99% - 109%
    EDTA Plasma 90 79% - 99%
    Cit plasma 94 88% - 98%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow

  • Product overview

    Mouse CD27 ELISA Kit (ab233619) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD27 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD27 with 10.9 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse CD27 ELISA Kit (ab233619)
    Other - Mouse CD27 ELISA Kit (ab233619)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of mouse CD27 standard curve in Sample Diluent NS
    Example of mouse CD27 standard curve in Sample Diluent NS

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native CD27 in mouse serum and plasma
    Interpolated concentrations of native CD27 in mouse serum and plasma

    The concentrations of CD27 were measured in duplicates, interpolated from the CD27 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 12.5%, plasma (heparin) 12.5%, and plasma (EDTA) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD27 concentration was determined to be 14.3 ng/mL in serum, 11.9 ng/mL in plasma (citrate), 13.4 g/ml in plasma (heparin), and 10.8 ng/ml in plasma (EDTA).

  • Interpolated concentrations of native CD27 in mouse EL4-IL2 cell culture supernatant samples, both untreated and treated with 10 µg/ml PHA and 10 ng/ml PMA for two days
    Interpolated concentrations of native CD27 in mouse EL4-IL2 cell culture supernatant samples, both untreated and treated with 10 µg/ml PHA and 10 ng/ml PMA for two days

    The concentrations of CD27 were measured in duplicates, interpolated from the CD27 standard curves and corrected for sample dilution. Undiluted samples are as follows: untreated EL4-IL2 supernatant 100% and treated EL4-IL2 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD27 concentration was determined to be 426 pg/mL in untreated EL4-IL2 supernatant and 1,978 pg/ml in treated EL4-IL2 supernatant.

  • Sandwich ELISA - Mouse CD27 ELISA Kit (ab233619)
    Sandwich ELISA - Mouse CD27 ELISA Kit (ab233619)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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