Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9731] to MiTF
Suitable for: WB
Reacts with: Mouse, Rat, Human

Product name

Anti-MiTF antibody [EPR9731]
See all MiTF primary antibodies
Description

Rabbit monoclonal [EPR9731] to MiTF
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment within Human MiTF. The exact sequence is proprietary.
Database link: O75030
Positive control
- WB: A-375, B16F0, mouse spleen, rat spleen and HeLa whole cell lysate (ab150035)
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9731
Isotype

IgG
Research areas

Western blot - Anti-MiTF antibody [EPR9731] (ab140606)

All lanes : Anti-MiTF antibody [EPR9731] (ab140606) at 1/200 dilution (purified)

Lane 1 : B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysates
Lane 2 : Rat spleen lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 58 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-MiTF antibody [EPR9731] (ab140606)

All lanes : Anti-MiTF antibody [EPR9731] (ab140606) at 1/1000 dilution (purified)

Lane 1 : A375 (Human malignant melanoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysates
Lane 4 : Mouse spleen lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 58 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-MiTF antibody [EPR9731] (ab140606)

All lanes : Anti-MiTF antibody [EPR9731] (ab140606) at 1/1000 dilution (unpurified)

Lane 1 : A-375 (Human malignant melanoma cell line) cell lysate
Lane 2 : B16F0 (Mouse melanoma cell line) cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 58 kDa
Observed band size: 52 kDa
why is the actual band size different from the predicted?
Anti-MiTF antibody [EPR9731] (ab140606)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com