Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21825] to CD2
Suitable for: WB, IHC-P, Flow Cyt, IP
Reacts with: Mouse

Product name

Anti-CD2 antibody [EPR21825]
See all CD2 primary antibodies
Description

Rabbit monoclonal [EPR21825] to CD2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
IHC-P	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse thymus lysate. IHC-P: Mouse thymus and spleen tissues. Flow Cyt: Mouse primary splenocytes. IP: Mouse thymus lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21825
Isotype

IgG
Research areas

Flow Cytometry - Anti-CD2 antibody [EPR21825] (ab219411)

Flow cytometric analysis of mouse primary splenocytes labeling CD2 with ab219411 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were surface stained with anti-mouse CD19-Alexa Fluor^®647 and rabbit IgG (left) / ab219411 (right). Gated on total viable cells.
Western blot - Anti-CD2 antibody [EPR21825] (ab219411)

Anti-CD2 antibody [EPR21825] (ab219411) at 1/1000 dilution + Mouse thymus lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 38 kDa
Observed band size: 45-58 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Molecular mass is consistent with the literature (PMID 10510361).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] (ab219411)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD2 with ab219411 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse spleen (PMID: 2901356) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] (ab219411)

Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD2 with ab219411 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse thymus (PMID: 2901356) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-CD2 antibody [EPR21825] (ab219411)

CD2 was immunoprecipitated from 0.35 mg of mouse thymus lysate with ab219411 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219411 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: Mouse thymus lysate 10 µg (Input).

Lane 2: ab219411 IP in mouse thymus lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219411 in mouse thymus lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.
Anti-CD2 antibody [EPR21825] (ab219411)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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