Neprilysin Assay Kit (Fluorometric) (ab241003)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
Overview
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Product name
Neprilysin Assay Kit (Fluorometric)
See all CD10 kits -
Detection method
Fluorescent -
Product overview
Neprilysin Assay Kit (Fluorometric) (ab241003) provides a quick and easy method for monitoring NEP activity in a wide variety of samples.
This kit utilizes the ability of an active NEP to cleave a synthetic substrate (Abz-based peptide) to release a free fluorophore. The released Abz can be easily quantified using a fluorescence microplate reader. The substrate is specific to NEP and can differentiate the NEP activity from Trypsin and other structurally similar zinc metalloproteinase in biological samples such as Angiotensin-Converting Enzymes (ACE1, ACE2), Endothelin Converting Enzymes (ECE1, ECE2).
This kit is simple, specific and can detect as low as 20 µU/mg of NEP activity.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Abz-Standard (1 mM) 1 x 100µl NEP Assay Buffer 1 x 40ml NEP Substrate (in DMSO) 1 x 15µl Neprilysin (Lyophilized) 1 vial -
Research areas
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Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. -
Sequence similarities
Belongs to the peptidase M13 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- Atriopeptidase
- CALLA
- CD10
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Images
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Abz-Standard Curve, results from multiple experiments.
Abz-Standard Curve, results from multiple experiments. (
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Measurement of purified NEP (10 ng), ACE1 (150 ng), ACE2 (0.5 ng), ECE1 (15 ng) and ECE2 (20 ng) activities using our proprietary substrate.Measurement of purified NEP (10 ng), ACE1 (150 ng), ACE2 (0.5 ng), ECE1 (15 ng) and ECE2 (20 ng) activities using our proprietary substrate. The kit can efficiently distinguish NEP activity from other zinc metalloproteases in biological samples.
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Measurement of NEP activityMeasurement of NEP activity in Rat Lung (10 µg protein) and Rat Kidney (8.5 µg protein). All assays were performed following kit protocol.