c-Myc Transcription Factor Assay Kit (Colorimetric) (ab207200)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr 30 min
- Sample type: Nuclear Extracts
- Sensitivity: 250 ng/well
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 5 x 96 tests 10X Antibody Binding Buffer 1 x 2.2ml 5 x 2.2ml 10X Wash Buffer 1 x 22ml 5 x 22ml 96-well c-Myc assay plate 1 unit 5 units Anti-rabbit HRP-conjugated IgG (0.25 μg/μL) 1 x 11µl 5 x 11µl Binding Buffer 1 x 10ml 5 x 10ml c-Myc antibody 1 x 11µl 5 x 11µl c-Myc Mutated oligonucleotide (10 pmol/μL) 1 x 100µl 5 x 100µl c-Myc Wild-type oligonucleotide (10 pmol/μL) 1 x 100µl 5 x 100µl Developing Solution 1 x 11ml 5 x 11ml Dithiothreitol (DTT) (1 M) 1 x 100µl 5 x 100µl Jurkat (1 day growth) nuclear extract (2.5 μg/μL) 1 x 40µl 5 x 40µl Lysis Buffer 1 x 10ml 5 x 10ml Plate sealer 1 unit 5 units Protease Inhibitor Cocktail 1 x 100µl 5 x 100µl Stop Solution 1 x 11ml 5 x 11ml -
Research areas
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Function
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. -
Involvement in disease
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsPhosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. -
Cellular localization
Nucleus > nucleoplasm. Nucleus > nucleolus. - Information by UniProt
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Alternative names
- AU016757
- Avian myelocytomatosis viral oncogene homolog
- bHLHe39
see all -
Database links
- Entrez Gene: 4609 Human
- Entrez Gene: 17869 Mouse
- Omim: 190080 Human
- SwissProt: P01106 Human
- SwissProt: P01108 Mouse
- Unigene: 202453 Human
- Unigene: 2444 Mouse
Images
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Different amounts of unstimulated Jurkat (Gray) and U-937 (Black) were tested for c-Myc activation. These results are provided for demonstration purposes only.
Different amounts of unstimulated U-937 (grey) and Jurkat (black) were tested for c-Myc activation. These results are provided for demonstration purposes only.