Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-n-Myc/MYCN antibody [EPR18982-13] - BSA and Azide free (ab250996)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18982-13] to n-Myc/MYCN - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-n-Myc/MYCN antibody [EPR18982-13] - BSA and Azide free
    See all n-Myc/MYCN primary antibodies

  • Description

    Rabbit monoclonal [EPR18982-13] to n-Myc/MYCN - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Detection of endogenous expression of n-Myc/MYCN in WB will need optimization. Under our experimental conditions we could not detect endogenous n-Myc/MYCN in human cell lysates.

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250996 is the carrier-free version of ab189528 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250996 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as n-Myc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-n-Myc/MYCN antibody [EPR18982-13] - BSA and Azide free (ab250996)
    Western blot - Anti-n-Myc/MYCN antibody [EPR18982-13] - BSA and Azide free (ab250996)
    All lanes : Anti-n-Myc/MYCN antibody [EPR18982-13] (ab189528) at 1/1000 dilution

    Lanes 1 & 3 & 5 : Untransfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate (control)
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with n-Myc/MYCN with GFP-tag
    Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with l-Myc with GST-tag
    Lane 6 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with c-Myc with His-tag

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Additional bands at: 83 kDa (possible tagged protein)


    Exposure time: 3 minutes


    This data was developed using ab189528, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    n-Myc/MYCN is tagged with GFP, therefore its expected MW is 83 kD under these conditions.

  • Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-13] - BSA and Azide free (ab250996)
    Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-13] - BSA and Azide free (ab250996)

    This data was developed using ab189528, the same antibody clone in a different buffer formulation.

    n-Myc/MYCN was immunoprecipitated from 2µg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates with ab194094 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab189528 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct, 10ug (Input).

    Lane 2: ab189528 IP in HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab189528 IP in HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    ab189528 immuno-precipitated the overexpressed n-Myc/MYCN-GFP (~100 kDa) in the transfected HEK-293 lysate and an additional 50 kDa off-target band. The overexpressed n-Myc/MYCN-GFP was recognized by ab290 (anti-GFP antibody, IP #2) and ab16898 (anti-n-Myc/MYCN antibody, IP #3).

  • Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-13] - BSA and Azide free (ab250996)
    Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-13] - BSA and Azide free (ab250996)
    This data was developed using ab189528, the same antibody clone in a different buffer formulation.n-Myc/MYCN was immunoprecipitated from 2μg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates with ab189528 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab16898 at 1/1000 dilution. Goat Anti-Mouse IgG (H+L) Peroxidase Conjugate, was used as secondary antibody at 1/10000 dilution. Lane 1: HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct, 10ug (Input). Lane 2: ab189528 IP in HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct. Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab189528 IP in HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second.
  • Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-13] - BSA and Azide free (ab250996)
    Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-13] - BSA and Azide free (ab250996)
    This data was developed using ab189528, the same antibody clone in a different buffer formulation.n-Myc/MYCN was immunoprecipitated from 2μg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates with ab189528 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab290 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Lane 1: HEK-293 whole cell lysate transfected with n-Myc/MYCN, 10ug (Input). Lane 2: ab189528 IP in HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct. Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab189528 IP in HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds.
  • Anti-n-Myc/MYCN antibody [EPR18982-13] - BSA and Azide free (ab250996)
    Anti-n-Myc/MYCN antibody [EPR18982-13] - BSA and Azide free (ab250996)

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