Anti-MiTF antibody [C5] (ab12039)
![Anti-MiTF antibody [C5] (ab12039)](https://www.abcam.com/ps/products/12/ab12039/Images/ab12039-19279-anti-mitf-antibody-c5-chip-grade-western-blot.jpg "Anti-MiTF antibody [C5] (ab12039)")
Key features and details
- Mouse monoclonal [C5] to MiTF
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-MiTF antibody [C5]
See all MiTF primary antibodies -
Description
Mouse monoclonal [C5] to MiTF -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment corresponding to Human MiTF (N terminal).
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Positive control
- WB: Malme-3M whole cell lysate. Flow Cyt: Malme-3M cells.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
C5 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-MiTF antibody [C5] (ab12039)Anti-MiTF antibody [C5] (ab12039) at 1 µg/ml + Malme 3m (Human melanoma cells) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 40,55,70 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
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![Flow Cytometry - Anti-MiTF antibody [C5] (ab12039)](https://www.abcam.com/ps/products/12/ab12039/Images/ab12039-19277-anti-mitf-antibody-c5-chip-grade-flow-cytometry.jpg)
Flow Cytometry - Anti-MiTF antibody [C5] (ab12039)Overlay histogram showing Malme 3m cells stained with ab12039 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12039, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
