Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p16 ARC antibody [EP1551Y] - BSA and Azide free (ab220800)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1551Y] to p16 ARC - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-p16 ARC antibody [EP1551Y] - BSA and Azide free
    See all p16 ARC primary antibodies

  • Description

    Rabbit monoclonal [EP1551Y] to p16 ARC - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody is specific to the p16 ARC protein, database link: O15511. There is no observed cross reactivity to the CDKN2A/p16INK4a protein.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, IP, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal, mouse and rat brain tissue lysates and HeLa, HepG2, SKBR-3 and MCF-7 cell lysates. IHC-P: Human uterus adenocarcinoma and spleen tissues. ICC/IF: Neuro-2a cells. IP: Human fetal brain tissue lysate.

  • General notes

    Ab220800 is the carrier-free version of ab51243. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab220800 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-p16 ARC antibody [EP1551Y] - BSA and Azide free (ab220800)
    Immunocytochemistry/ Immunofluorescence - Anti-p16 ARC antibody [EP1551Y] - BSA and Azide free (ab220800)

    Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling p16 ARC with purified ab51243 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51243).

  • Immunoprecipitation - Anti-p16 ARC antibody [EP1551Y] - BSA and Azide free (ab220800)
    Immunoprecipitation - Anti-p16 ARC antibody [EP1551Y] - BSA and Azide free (ab220800)

    ab51243 (purified) at a dilution of 1/20 immunoprecipitating p16 ARC in human fetal brain tissue lysate.

    Lane 1 (input): Human fetal brain tissue lysate (10µg)

    Lane 2 (+): ab51243 + human fetal brain tissue lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51243 in human fetal brain tissue lysate.

    For western blotting, a HRP-conjugated anti-rabbit specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51243).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p16 ARC antibody [EP1551Y] - BSA and Azide free (ab220800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p16 ARC antibody [EP1551Y] - BSA and Azide free (ab220800)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling p16 ARC with purified ab51243 at a dilution of 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51243).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p16 ARC antibody [EP1551Y] - BSA and Azide free (ab220800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p16 ARC antibody [EP1551Y] - BSA and Azide free (ab220800)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus adenocarcinoma tissue labelling p16 ARC with unpurified ab51243 at a dilution of 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51243).

  • Anti-p16 ARC antibody [EP1551Y] - BSA and Azide free (ab220800)
    Anti-p16 ARC antibody [EP1551Y] - BSA and Azide free (ab220800)

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