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Immunology Adaptive Immunity T Cells CD

Human CD23 Antibody Pair - BSA and Azide free (ab253355)

Human CD23 Antibody Pair - BSA and Azide free (ab253355)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

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Overview

  • Product name

    Human CD23 Antibody Pair - BSA and Azide free
    See all CD23 kits
  • Assay type

    ELISA set
  • Range

    37.5 pg/ml - 2400 pg/ml
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human CD23. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab272468), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human CD23 Capture Antibody (unconjugated) 1 x 100µg
    Human CD23 Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Immunoglobulins
    • Receptors
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
  • Function

    This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Cell membrane. Secreted. Also exists as a soluble excreted form.
  • Target information above from: UniProt accession P06734 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Blast 2
    • BLAST-2
    • Blast2
    • C type lectin domain family 4 member J
    • C-type lectin domain family 4
    • C-type lectin domain family 4 member J
    • C-type lectin domain family 4, member J
    • CD 23
    • CD 23A
    • CD23
    • CD23 antigen
    • CD23A
    • CLEC 4J
    • CLEC4J
    • Fc epsilon receptor II
    • Fc epsilon RII
    • Fc fragment of IgE
    • Fc fragment of IgE low affinity II receptor for
    • Fc fragment of IgE receptor II
    • Fc fragment of IgE, low affinity II, receptor for (CD23)
    • Fc of IgE
    • Fc of IgE, low affinity II, receptor for (CD23)
    • Fc receptor IgE low affinity II alpha polypeptide
    • Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a
    • Fc-epsilon-RII
    • FCE 2
    • FCE2
    • FCER 2
    • Fcer2
    • FCER2_HUMAN
    • FCER2A
    • FceRII
    • IgE binding factor
    • IgE receptor lymphocyte
    • IgE-binding factor
    • IGEBF
    • Immunoglobulin E binding factor
    • Immunoglobulin E receptor
    • Immunoglobulin E receptor, low affinity II
    • Immunoglobulin E-binding factor
    • Immunoglobulin epsilon chain
    • LEUKOCYTE ANTIGEN CD23
    • Low Affinity IgE Receptor
    • Low affinity immunoglobulin epsilon Fc receptor
    • Low affinity immunoglobulin epsilon Fc receptor membrane bound form
    • Low affinity immunoglobulin epsilon Fc receptor soluble form
    • Ly-42
    • Ly42
    • Lymphocyte antigen CD23
    • Lymphocyte IgE receptor
    • MGC93219
    see all
  • Database links

    • Entrez Gene: 2208 Human
    • Omim: 151445 Human
    • SwissProt: P06734 Human
    • Unigene: 465778 Human

    Images

    • Sandwich ELISA - Human CD23 Antibody Pair - BSA and Azide free (ab253355)
      Sandwich ELISA - Human CD23 Antibody Pair - BSA and Azide free (ab253355)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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