Human CD23 ELISA Kit (ab272468)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 12 pg/ml
- Range: 37.5 pg/ml - 2400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD23 ELISA Kit
See all CD23 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 7 3.3% Inter-assay Sample n Mean SD CV% Serum 3 4.4% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
12 pg/ml -
Range
37.5 pg/ml - 2400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 106 98% - 116% Serum 92 85% - 98% Hep Plasma 101 100% - 102% EDTA Plasma 105 99% - 113% Cit plasma 92 91% - 93% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD23 ELISA kit (ab272199) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD23 protein in serum, plasma, urine and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD23 with 12 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CD23, also known as Low Affinity Immunoglobulin Epsilon Fc Receptor, is a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. It has essential roles in the regulation of IgE production and in the differentiation of B-cells. It is a B-cell-specific antigen. CD23 is a 47 kDa type II transmembrane glycoprotein containing a short N-terminal cytoplasmic tail, a transmembrane domain, and a large C-terminal extracellular domain containing C-type lectin domain. A soluble CD23 form also exists.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD23 Capture Antibody 1 x 600µl 10X Human CD23 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human CD23 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane. Secreted. Also exists as a soluble excreted form. - Information by UniProt
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Alternative names
- Blast 2
- BLAST-2
- Blast2
see all -
Database links
- Entrez Gene: 2208 Human
- Omim: 151445 Human
- SwissProt: P06734 Human
- Unigene: 465778 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CD23 standard curve in Sample Diluent NS.The CD23 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CD23 in human serum and plasma samples.The concentrations of CD23 were measured in duplicates, interpolated from the CD23 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 25%, plasma (EDTA) 100% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD23 concentration was determined to be 1,741 pg/mL in neat serum, 1,601 pg/mL in neat plasma (citrate), 1,476 pg/mL in neat plasma (EDTA) and 1,930 pg/mL in neat plasma (heparin).
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Interpolated concentrations of native CD23 in human urine, Raji cell culture supernatant and PBMC cell culture supernatant samples treated with or without 1.5% PHA for 36 hours.The concentrations of CD23 were measured in duplicates, interpolated from the CD23 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 25%, Raji supernatant 50%, PBMC PHA treated supernatant 7% and PBMC untreated supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD23 concentration was determined to be 4,289 pg/mL in neat urine, 4,358 pg/mL in neat Raji supernatant, 23,923 pg/mL in neat PBMC PHA treated supernatant and 498 pg/mL in PBMC untreated supernatant.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD23 concentration was determined to be 2,495 pg/mL with a range of 1,609 – 4,772 pg/mL.
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Sandwich ELISA - Human CD23 ELISA Kit (ab272468)To learn more about the advantages of recombinant antibodies see here.
