Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202]
See all Tripeptidyl peptidase II/TPPII primary antibodies -
Description
Rabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
Recombinant fragment within Human Tripeptidyl peptidase II/TPPII. The exact sequence is proprietary.
Database link: P29144 -
Positive control
- Jurkat, HeLa, HepG2, and fetal liver lysates; Human tonsil tissue; HeLa cells; Jurkat cells.
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General notes
This product was previously labelled as Tripeptidyl peptidase II
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13202 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177) at 1/1000 dilution
Lane 1 : Jurkat lysate
Lane 2 : HeLa lysate
Lane 3 : HepG2 lysate
Lane 4 : fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 138 kDa
Additional bands at: 138 kDa. We are unsure as to the identity of these extra bands.
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Flow Cytometrical analysis of permeabilized Jurkat cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/10 (red) or a rabbit IgG (negative) (green).
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Immunofluorescent analysis of HeLa cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/100 (red) and DAPI staining (blue).
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Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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