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Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3968(2)] to TEF1/TEAD-1
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-TEF1/TEAD-1 antibody [EPR3968(2)]
    See all TEF1/TEAD-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3968(2)] to TEF1/TEAD-1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    within Human TEF1/TEAD-1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • A549, HeLa, A431, 293T, and fetal muscle lysates; and HeLa cells.

  • General notes

     

     

    Previously labelled as TEF1. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535)
    Western blot - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535)
    All lanes : Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : TEAD1 knockout A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 2: Merged signal (red and green). Green - ab133535 observed at 50 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab133535 was shown to react with TEF1/TEAD-1 in wild-type A549 cells in western blot with loss of signal observed in TEAD1 knockout sample. Wild-type and TEAD1 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab133535 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535)
    Immunocytochemistry/ Immunofluorescence - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535)

    Immunofluorescence analysis of HeLa cells labelling TEF1/TEAD-1 with ab133535 at 1/50

  • Western blot - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535)
    Western blot - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535)
    All lanes : Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535) at 1/1000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : A431 cell lysates
    Lane 3 : 293T cell lysates
    Lane 4 : Fetal muscle lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 48 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535)
    OI-RD Scanning - Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535)
    Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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