Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR039F] to IRF1
Suitable for: WB, ICC, IHC-P, IP
Reacts with: Human

Product name

Anti-IRF1 antibody [BLR039F]
See all IRF1 primary antibodies
Description

Rabbit monoclonal [BLR039F] to IRF1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human IRF1 aa 250-300. The exact sequence is proprietary. NP_002189.1 and Gene ID 3659.
Database link: P10914
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR039F
Isotype

IgG
Research areas

Immunoprecipitation - Anti-IRF1 antibody [BLR039F] (ab243895)

IRF1 was immunoprecipitated from 1.0 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab243895 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243895 at 1/1000 dilution.
Lane 1: ab243895 IP in Jurkat whole cell lysate.

Lane 2: Control IgG in Jurkat whole cell lysate.

Detection: Chemiluminescence with an exposure time of 30 seconds.
Western blot - Anti-IRF1 antibody [BLR039F] (ab243895)

Western blot analysis using ab243895 at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate (50 µg).
Lane 2: HepG2 whole cell lysate (50 µg).
Lane 3: HeLA (+ IFN-gamma) whole cell lysate (50 µg).
Lane 4: HeLa whole cell lysate (50 µg).
Lane 5: SR whole cell lysate (50 µg).
Lane 6: MOLT-4 whole cell lysate (50 µg).
Lane 7: RPMI 8226 whole cell lysate (50 µg).
Lane 8: KG-1 whole cell lysate (50 µg).
Lane 9: A549 whole cell lysate (50 µg).
Lane 10: MCF7 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 seconds.
Immunocytochemistry - Anti-IRF1 antibody [BLR039F] (ab243895)

Formalin-fixed, paraffin-embedded OVCAR-8 cells labeling IRF1 using ab243895 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF1 antibody [BLR039F] (ab243895)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for IRF1 using ab243895 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Anti-IRF1 antibody [BLR039F] (ab243895)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com