Anti-IRF1 antibody (ab245338)
Key features and details
- Rabbit polyclonal to IRF1
- Suitable for: WB, IP
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-IRF1 antibody
See all IRF1 primary antibodies -
Description
Rabbit polyclonal to IRF1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse
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Immunogen
Synthetic peptide within Mouse IRF1 aa 200-250. The exact sequence is proprietary. (NP_032416.1).
Database link: P15314 -
Positive control
- WB: NIH/3T3 whole cell lysate. IP: NIH/3T3 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245338 was affinity purified using an epitope specific to IRF1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IRF1 antibody (ab245338)All lanes : Anti-IRF1 antibody (ab245338) at 0.2 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Lane 2 : NIH/3T3 whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 1 minute
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Immunoprecipitation - Anti-IRF1 antibody (ab245338)IRF1 was immunoprecipitated from NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245338 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245338 at 1 µg/ml.
Lane 1: ab245338 IP in NIH/3T3 whole cell lysate.
Lane 2: Control IgG IP in NIH/3T3 whole cell lysate.Detection: Chemiluminescence with exposure time of 60 seconds.
