Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3967(2)] to TEF1/TEAD-1 - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free
    See all TEF1/TEAD-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3967(2)] to TEF1/TEAD-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • A549, HeLa, 293T, and fetal muscle lysates; Human placenta and skeletal muscle tissue. L6 (Rat skeletal muscle myoblast) and C2C12 whole cell lysates.

  • General notes

    Ab219647 is the carrier-free version of ab133533. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab219647 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)
    Western blot - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)
    All lanes : Anti-TEF1/TEAD-1 antibody [EPR3967(2)] (ab133533) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : TEAD1 knockout A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab133533).

    Lanes 1 - 2: Merged signal (red and green). Green - ab133533 observed at 50 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab133533 was shown to react with TEF1/TEAD-1 in wild-type A549 cells in western blot with loss of signal observed in TEAD1 knockout sample. Wild-type and TEAD1 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab133533 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)
    Immunoprecipitation - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

    ab133533 (purified) at 1:80 dilution (2ug) immunoprecipitating TEF1/TEAD-1 in 293 (Human embryonic kidney epithelial cell) whole cell lysate.
    Lane 1 (input): 293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab133533 & 293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133533 in 293 (Human embryonic kidney epithelial cell) whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133533).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling TEF1/TEAD-1 with Purified  ab133533 at 1:1000 dilution (1.63 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133533).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling TEF1/TEAD-1 with Purified  ab133533 at 1:1000 dilution (1.63 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133533).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue sections labeling TEF1/TEAD-1 with Purified ab133533 at 1:1000 dilution (1.63 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133533).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

    Immunohistochemistry analysis of Paraffin Embedded Human placenta tissue labelling TEF1/TEAD-1 with unpurified ab133533 at 1/100. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133533).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

    Immunohistochemistry analysis of Paraffin Embedded Human skeletal muscle tissue labelling TEF1/TEAD-1 with unpurified ab133533 at 1/100. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133533).

  • OI-RD Scanning - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)
    OI-RD Scanning - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133533).

  • Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)
    Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

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