Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HNF1 alpha antibody [EPR23054-108] - BSA and Azide free (ab272708)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23054-108] to HNF1 alpha - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HNF1 alpha antibody [EPR23054-108] - BSA and Azide free
    See all HNF1 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR23054-108] to HNF1 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
    Unsuitable for: ChIP or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, Caco-2 and Hepa1-6 whole cell lysate; Mouse liver lysate; Rat liver lysate. IHC-P: Human liver and colon tissues. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.

  • General notes

    ab272708 is the carrier-free version of ab272693. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-HNF1 alpha antibody [EPR23054-108] - BSA and Azide free (ab272708)
    Flow Cytometry - Anti-HNF1 alpha antibody [EPR23054-108] - BSA and Azide free (ab272708)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HepG2 (Human hepatocellular carcinoma epithelial cell, Right) cells labelling HNF1 alpha with ab272693 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HeLa (PMID: 10677375).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272693).

  • Immunocytochemistry/ Immunofluorescence - Anti-HNF1 alpha antibody [EPR23054-108] - BSA and Azide free (ab272708)
    Immunocytochemistry/ Immunofluorescence - Anti-HNF1 alpha antibody [EPR23054-108] - BSA and Azide free (ab272708)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HepG2 and HeLa cells labelling HNF1 alpha with ab272693 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HepG2 cells. 100% methanol fixation is recommended. Negative control: HeLa (PMID: 10677375). ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000  dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272693).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [EPR23054-108] - BSA and Azide free (ab272708)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [EPR23054-108] - BSA and Azide free (ab272708)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HNF1 alpha with ab272693 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human colon. The section was incubated with ab272693 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272693).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [EPR23054-108] - BSA and Azide free (ab272708)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF1 alpha antibody [EPR23054-108] - BSA and Azide free (ab272708)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HNF1 alpha with ab272693 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human liver. The section was incubated with ab272693 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272693).

  • Anti-HNF1 alpha antibody [EPR23054-108] - BSA and Azide free (ab272708)
    Anti-HNF1 alpha antibody [EPR23054-108] - BSA and Azide free (ab272708)

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