Anti-DACH1 antibody (ab226176)
Key features and details
- Rabbit polyclonal to DACH1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DACH1 antibody
See all DACH1 primary antibodies -
Description
Rabbit polyclonal to DACH1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human DACH1 aa 740-760. The exact sequence is proprietary.
Database link: Q9UI36 -
Positive control
- IHC-P: Human breast carcinoma tissue. WB: HEK-293T whole cell lysate. IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226176 was affinity purified using an epitope specific to DACH1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DACH1 antibody (ab226176)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for DACH1 using ab226176 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: Hematoxylin (blue).
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Western blot - Anti-DACH1 antibody (ab226176)All lanes : Anti-DACH1 antibody (ab226176) at 0.04 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 79 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-DACH1 antibody (ab226176)DACH1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226176 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226176 at 1 µg/ml.
Lane 1: ab226176 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
