Anti-Brd4 antibody (ab223223)
Key features and details
- Goat polyclonal to Brd4
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Brd4 antibody
See all Brd4 primary antibodies -
Description
Goat polyclonal to Brd4 -
Host species
Goat -
Specificity
ab223223 is expected to recognize both reported isoforms (NP_490597.1; NP_055114.1). -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Human Brd4 aa 1-100 (internal sequence) (Cysteine residue). (NP_490597.1).
Database link: O60885 -
Positive control
- WB: Mouse testis lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab223223 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Brd4 antibody (ab223223)Anti-Brd4 antibody (ab223223) at 1 µg/ml + Mouse testis lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 152 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
