Anti-CPT1B antibody (ab134988)
Key features and details
- Rabbit polyclonal to CPT1B
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CPT1B antibody
See all CPT1B primary antibodies -
Description
Rabbit polyclonal to CPT1B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Horse, Dog, Pig, Chimpanzee, Macaque monkey
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Immunogen
Synthetic peptide corresponding to Human CPT1B aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: Q92523 -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Heart; Human Fetal Heart; Human Skeletal Muscle. IHC-P: Human heart FFPE tissue sections.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa). Target
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Tissue specificity
Strong expression in heart and skeletal muscle. No expression in liver and kidney. -
Pathway
Lipid metabolism; fatty acid beta-oxidation. -
Sequence similarities
Belongs to the carnitine/choline acetyltransferase family. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 1375 Human
- Entrez Gene: 12895 Mouse
- Omim: 601987 Human
- SwissProt: Q92523 Human
- SwissProt: Q924X2 Mouse
- SwissProt: Q8HY46 Pig
- Unigene: 439777 Human
- Unigene: 609867 Human
see all -
Alternative names
- muscle isoform antibody
- Carnitine O palmitoyltransferase 1B antibody
- Carnitine O palmitoyltransferase I mitochondrial muscle isoform antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1B antibody (ab134988)
IHC image of CPT1B staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134988, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-CPT1B antibody (ab134988)All lanes : Anti-CPT1B antibody (ab134988) at 1 µg/ml (Milk blocking 3%)
Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue
Lane 2 : Heart (Human) Whole Cell Lysate - fetal normal tissue
Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue
Lane 4 : Liver (Human) Tissue Lysate - adult normal tissue (Negative control)
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 87 kDa
Exposure time: 4 minutesPlease note that Human Liver tissue lysate in Lane 4 was used as a negative control.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab134988 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
References (9)
ab134988 has been referenced in 9 publications.
- Ma Y et al. Functional analysis of molecular and pharmacological modulators of mitochondrial fatty acid oxidation. Sci Rep 10:1450 (2020). PubMed: 31996743
- He J et al. Epimedium Polysaccharide Ameliorates Benzene-Induced Aplastic Anemia in Mice. Evid Based Complement Alternat Med 2020:5637507 (2020). PubMed: 32256652
- Wu D et al. Prohibitin 2 deficiency impairs cardiac fatty acid oxidation and causes heart failure. Cell Death Dis 11:181 (2020). PubMed: 32165613
- Hoffman NJ et al. Genetic loss of AMPK-glycogen binding destabilises AMPK and disrupts metabolism. Mol Metab 41:101048 (2020). PubMed: 32610071
- Ferchaud-Roucher V et al. Maternal obesity results in decreased syncytiotrophoblast synthesis of palmitoleic acid, a fatty acid with anti-inflammatory and insulin-sensitizing properties. FASEB J 33:6643-6654 (2019). PubMed: 30811959
- Leger T et al. Dietary EPA Increases Rat Mortality in Diabetes Mellitus, A Phenomenon Which Is Compensated by Green Tea Extract. Antioxidants (Basel) 8:N/A (2019). PubMed: 31690052
- Yang Y et al. Chinese patent medicine Xin-Ke-Shu inhibits Ca2+ overload and dysfunction of fatty acid ß-oxidation in rats with myocardial infarction induced by LAD ligation. J Chromatogr B Analyt Technol Biomed Life Sci 1079:85-94 (2018). PubMed: 29454926
- Eleftheriadis T et al. IDO decreases glycolysis and glutaminolysis by activating GCN2K, while it increases fatty acid oxidation by activating AhR, thus preserving CD4+ T-cell survival and proliferation. Int J Mol Med 42:557-568 (2018). PubMed: 29693118
- Eleftheriadis T et al. Indoleamine 2,3-dioxygenase, by degrading L-tryptophan, enhances carnitine palmitoyltransferase I activity and fatty acid oxidation, and exerts fatty acid-dependent effects in human alloreactive CD4+ T-cells. Int J Mol Med 38:1605-1613 (2016). PubMed: 27667153
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1B antibody (ab134988)
IHC image of CPT1B staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134988, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
Western blot - Anti-CPT1B antibody (ab134988)All lanes : Anti-CPT1B antibody (ab134988) at 1 µg/ml (Milk blocking 3%)
Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue
Lane 2 : Heart (Human) Whole Cell Lysate - fetal normal tissue
Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue
Lane 4 : Liver (Human) Tissue Lysate - adult normal tissue (Negative control)
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 87 kDa
Exposure time: 4 minutesPlease note that Human Liver tissue lysate in Lane 4 was used as a negative control.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab134988 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
