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Rat FLT1 ELISA Kit (VEGF-R1) (ab270206)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 0.11 ng/ml
  • Range: 0.188 ng/ml - 12 ng/ml
  • Sample type: Cell culture supernatant, EDTA Plasma
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

Overview

  • Product name

    Rat FLT1 ELISA Kit (VEGF-R1)
    See all VEGF Receptor 1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Supernatant 8 5%
    Inter-assay
    Sample n Mean SD CV%
    Supernatant 3 10.5%

  • Sample type

    Cell culture supernatant, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.11 ng/ml

  • Range

    0.188 ng/ml - 12 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 118 117% - 121%
    EDTA Plasma 98 95% - 103%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Rat

  • Product overview

    Rat FLT1 ELISA Kit (VEGF-R1) (ab270206) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FLT1 (VEGF-R1) protein in edta plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat FLT1 (VEGF-R1) with 0.11 ng/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Fms-like tyrosine kinase 1 (FLT1), also known as vascular endothelial growth factor receptor 1 (VEGFR1), is a Tyrosine kinase encoded by the gene Flt1. VEGFR1 is expressed as a 1316 amino acid type 1 membrane glycoprotein by vascular endothelial cells, placental trophoblasts, and peripheral blood monocytes. VEGFR1 acts as cell-surface receptor for VEGFR-A, VEGFR-B, and placental growth factor (PGF) and may have an essential role in the development of embryonic vasculature by inhibiting excessive proliferation of endothelial cells.  

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of rat FLT1 standard curve in Sample Diluent 50BP.
    Example of rat FLT1 standard curve in Sample Diluent 50BP.

    The FLT1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native FLT1 in rat lung supernatant.
    Interpolated concentrations of native FLT1 in rat lung supernatant.

    The concentrations of FLT1 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: Lung supernatant (100%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 3.2 ng/mL in lung cell supernatant.

  • Sandwich ELISA - Rat FLT1 ELISA Kit (VEGF-R1) (ab270206)
    Sandwich ELISA - Rat FLT1 ELISA Kit (VEGF-R1) (ab270206)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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