Mouse HE4 ELISA Kit (ab245724)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.99 pg/ml
- Range: 7.81 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse HE4 ELISA Kit
See all HE4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% protein 8 4.1% -
Sample type
Cell culture supernatant -
Assay type
Sandwich (quantitative) -
Sensitivity
1.99 pg/ml -
Range
7.81 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 90 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse HE4 ELISA Kit (ab245724) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HE4 protein in cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse HE4 with 1.99 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse HE4 Capture Antibody 1 x 600µl 10X Mouse HE4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse HE4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Tissue specificity
Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. -
Sequence similarities
Contains 2 WAP domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- dJ461P17.6
- EDDM4
- epididymal protein 4
see all -
Database links
- Entrez Gene: 67701 Mouse
- SwissProt: Q9DAU7 Mouse
- Unigene: 27289 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse HE4 standard curve in Sample Diluent NS.The HE4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Sandwich ELISA - Mouse HE4 ELISA Kit (ab245724)To learn more about the advantages of recombinant antibodies see here.
