Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)
![Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)](https://www.abcam.com/ps/products/1/ab1927/Images/ab1927-331323-mouse-monoclonal-2c11-anti-human-igg1-fc-elisa.jpg "Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)")
Key features and details
- Mouse monoclonal [2C11] Anti-Human IgG1 Fc
- Host species: Mouse
- Isotype: IgG1
- Suitable for: IHC-Fr, ELISA
Overview
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Product name
Mouse monoclonal [2C11] Anti-Human IgG1 Fc
See all IgG1 secondary antibodies -
Host species
Mouse -
Target species
Human -
Tested applications
Suitable for: IHC-Fr, ELISAmore details -
Immunogen
IgG from human serum.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Chromatography on protein A Sepharose -
Clonality
Monoclonal -
Clone number
2C11 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
General notes
Concentration varies from lot to lot and can be provided on request. -
Research areas
Images
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ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927) Marino et al PLoS One. 2017 May 18;12(5):e0177810. doi: 10.1371/journal.pone.0177810. eCollection 2017. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Characterisation of virus activity and functional expression of monoclonal antibodies.
(Panel B) IgG1 antibody expression detected in cellular supernatants of HT-29 cells infected with 10ppc NG-135 by anti-IgG1 ELISA.
Media was removed from infected or control cells, clarified by centrifugation and diluted 1 in 2 in 3% BSA/PBS for storage at -20°C. For quantification of human IgG1, 96 well plates (NUNC) were coated overnight, 4°C, with mouse monoclonal anti-human IgG1 Fc antibody (ab1927, Abcam) at a 1:1000 dilution in carbonate/bicarbonate buffer. Plates were blocked in 3% BSA/PBS, washed and samples and standards added to the plate for 1 hr and RT. Secondary detection was carried out using a HRP conjugated goat anti-human IgG Fab (ab87422, Abcam) incubated for 1 hr, RT.