Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

500 µg 271 382 ₸ 500 µg 271 382 ₸

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Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007)

Key features and details

Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed
Conjugation: PE. Ex: 488nm, Em: 575nm
Host species: Donkey
Isotype: IgG
Suitable for: IHC-P, ICC/IF, Immunomicroscopy, Flow Cyt

Product name

Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed
See all IgG secondary antibodies
Description

Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed
Host species

Donkey
Target species

Rabbit
Tested applications

Suitable for: IHC-P, ICC/IF, Immunomicroscopy, Flow Cytmore details
Minimal
cross-reactivity

Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
more details
Immunogen

Rabbit IgG whole molecule
Conjugation

PE. Ex: 488nm, Em: 575nm

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

Prepared from monospecific antiserum by immunoaffinity chromatography using rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins
Conjugation notes

R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD_566nm/OD_280nm: 2.8
Clonality

Polyclonal
Isotype

IgG
General notes

Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Research areas

Flow Cytometry - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007)

Ab7007 was used with the primary antibody ab18203 in Flow Cyt. See Abreview on ab18203.
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007)

Ab7007 was used at dilution 1/250 with the primary antibody ab24182 in ICC/IF. See the review on ab24182.

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