Anti-CAMKIV antibody (ab235058)
Key features and details
- Rabbit polyclonal to CAMKIV
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CAMKIV antibody
See all CAMKIV primary antibodies -
Description
Rabbit polyclonal to CAMKIV -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human CAMKIV aa 300 to the C-terminus.
Database link: Q16566 -
Positive control
- WB: HEK-293T cell lysate. IP: Jurkat whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CAMKIV antibody (ab235058)Anti-CAMKIV antibody (ab235058) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
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Immunoprecipitation - Anti-CAMKIV antibody (ab235058)CAMKIV was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab235058. Western blot was performed from the immunoprecipitate using ab235058 at 1/200 dilution. HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody at 1/50000 dilution.
Lane 1: 1 µg rabbit monoclonal IgG instead of ab235058 in Jurkat whole cell lysate.
Lane 2: ab235058 IP in Jurkat whole cell lysate.
Lane 3: Jurkat whole cell lysate 20 µg (Input).
