Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CAMKIV antibody [EP2565AY] (ab68218)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2565AY] to CAMKIV
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Human

Product name

Anti-CAMKIV antibody [EP2565AY]
See all CAMKIV primary antibodies
Description

Rabbit monoclonal [EP2565AY] to CAMKIV
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

A synthetic peptide corresponding to residues near the C-terminus of human CAMKIV.
Positive control
- Human brain tissue sections, human ovarian adenocarcinoma tissue sections and SH SY5Y, Jurkat cell lysate. IF/ICC: HeLa cell line.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP2565AY
Isotype

IgG
Research areas

Western blot - Anti-CAMKIV antibody [EP2565AY] (ab68218)

All lanes : Anti-CAMKIV antibody [EP2565AY] (ab68218) at 1/1000000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : SH-SY5Y cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CAMKIV antibody [EP2565AY] (ab68218)

ab68218 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68218 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKIV antibody [EP2565AY] (ab68218)

ab68218 at 1/250-1/500 dilution staining CAMKIV in human brain tissue sections.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKIV antibody [EP2565AY] (ab68218)

ab68218 at 1/250-1/500 dilution staining CAMKIV in human ovarian adenocarcinoma tissue sections.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-CAMKIV antibody [EP2565AY] (ab68218)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com