Nivolumab ELISA Kit (Opdivo®) (ab237651)
Key features and details
- Sensitivity: 10 ng/ml
- Range: 30 ng/ml - 3000 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Nivolumab ELISA Kit (Opdivo®)
See all Nivolumab kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10 ng/ml -
Range
30 ng/ml - 3000 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Nivolumab ELISA Kit (Opdivo®) (ab237651) has been developed for specific quantification of Nivolumab concentration in human serum or plasma with high sensitivity and reproducibility. The density of color is proportional to the amount of Omalizumab captured from the samples and can be quantified when compared with standard curve.
Nivolumab (Opdivo®) is a human immunoglobulin G4 (IgG4) monoclonal antibody that binds to the programmed cell death 1 (PD-1) receptor and selectively blocks interaction with its programmed death ligands PD-L1 and PD-L2. Upregulation of PD-1 ligands occurs in some tumors and signalling through this pathway can contribute to inhibition of active T-cell immune surveillance of tumour tissue. The inhibitory effect of PD-1 and its ligands occurs through the promotion of apoptosis in antigen specific T cells while simultaneously blocking apoptosis in suppressor T cells. Blocking PD-1 activity has been shown to lead to decreased tumour growth in mouse tumour models.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests Micro ELISA Plate 1 unit Nivolumab Standard S1 1 x 0.3ml Nivolumab Standard S2 1 x 0.3ml Nivolumab Standard S3 1 x 0.3ml Nivolumab Standard S4 1 x 0.3ml Nivolumab Standard S5 1 x 0.3ml Nivolumab Standard S6 1 x 0.3ml Nivolumab Standard S7 1 x 0.3ml Nivolumab Standard S8 1 x 0.3ml Assay Buffer 2 x 50ml HRP-conjugate Probe 1 x 12ml TMB substrate 1 x 12ml Stop Solution 1 x 12ml Wash buffer (20X) 1 x 50ml Plate sealers 2 units -
Relevance
Nivolumab (Opdivo®) is a human immunoglobulin G4 (IgG4) monoclonal antibody that binds to the programmed cell death 1 (PD-1) receptor and selectively blocks interaction with its programmed death ligands PD-L1 and PD-L2. Upregulation of PD-1 ligands occurs in some tumors and signalling through this pathway can contribute to inhibition of active T-cell immune surveillance of tumour tissue. The inhibitory effect of PD-1 and its ligands occurs through the promotion of apoptosis in antigen specific T cells while simultaneously blocking apoptosis in suppressor T cells. Blocking PD-1 activity has been shown to lead to decreased tumour growth in mouse tumour models. -
Alternative names
- Opdivo
Images
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Example of Nivolumab standard curve
Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.