Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-beta Catenin non-phospho(active) S37/T41 antibody [EPR23969-131] - BSA and Azide free (ab278503)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23969-131] to beta Catenin non-phospho(active) S37/T41 - BSA and Azide free
  • Suitable for: Dot blot, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-beta Catenin non-phospho(active) S37/T41 antibody [EPR23969-131] - BSA and Azide free
    See all beta Catenin non-phospho(active) S37/T41 primary antibodies

  • Description

    Rabbit monoclonal [EPR23969-131] to beta Catenin non-phospho(active) S37/T41 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, IP, WBmore details
    Unsuitable for: ChIP,Flow Cyt (Intra),ICC,IHC-Fr or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HeLa, HAP1, HCT 116, LLC1, C6 and NIH/3T3, PC-12 lysates. IP: HeLa cell. Dot Blot: beta Catenin non-phospho-Ser33/Ser37/Thr41 peptide (aa 29-44), (aa 31-47).

  • General notes

    Non-phospho(active) Ser37/Thr41

    ab278503 is the carrier-free version of ab246504. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278503 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23969-131

  • Isotype

    IgG

Images

  • Western blot - Anti-beta Catenin antibody [EPR23969-131] - BSA and Azide free (ab278503)
    Western blot - Anti-beta Catenin antibody [EPR23969-131] - BSA and Azide free (ab278503)
    All lanes : Anti-beta Catenin non-phospho(active) S37/T41 antibody [EPR23969-131] (ab246504) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : CTNNB1 (beta Catenin) knockout HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate
    Lane 4 : CTNNB1 (beta Catenin) knockout HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/10000 dilution

    Predicted band size: 85 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab246504, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 16288032).

    Lanes 1-4: Merged signal (red and green). Green - ab246504 observed at 92 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

    Lanes 1-2: ab246504 Anti-beta Catenin (non-phospho(active) S37/T41) antibody [EPR23969-131] was shown to specifically react with beta Catenin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255352 (knockout cell lysate ab263756) was used.

    Lanes 3-4: ab246504 Anti-beta Catenin (non-phospho(active) S37/T41) antibody [EPR23969-131] was shown to specifically react with beta Catenin in wild-type HAP1 cells. Loss of signal was observed when knockout sample was used.

    Wild-type and beta Catenin knockout samples were subjected to SDS-PAGE. ab246504 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

    An extra band around 45 kDa was observed.

     

  • Western blot - Anti-beta Catenin antibody [EPR23969-131] - BSA and Azide free (ab278503)
    Western blot - Anti-beta Catenin antibody [EPR23969-131] - BSA and Azide free (ab278503)
    All lanes : Anti-beta Catenin non-phospho(active) S37/T41 antibody [EPR23969-131] (ab246504) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate
    Lane 3 : LLC1 (mouse lung carcinoma ) whole cell lysate
    Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 85 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?



    This data was developed using ab246504, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration:  5% NFDM/TBST

    Exposure time 92 seconds

    An extra band around 45 kDa was observed.

  • Western blot - Anti-beta Catenin antibody [EPR23969-131] - BSA and Azide free (ab278503)
    Western blot - Anti-beta Catenin antibody [EPR23969-131] - BSA and Azide free (ab278503)
    All lanes : Anti-beta Catenin non-phospho(active) S37/T41 antibody [EPR23969-131] (ab246504) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 85 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?



    This data was developed using ab246504, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    An extra band around 45 kDa was observed.

    Exposure time: 3 minutes

     

     

  • Dot Blot - Anti-beta Catenin antibody [EPR23969-131] - BSA and Azide free (ab278503)
    Dot Blot - Anti-beta Catenin antibody [EPR23969-131] - BSA and Azide free (ab278503)

    This data was developed using ab246504, the same antibody clone in a different buffer formulation.

    Dot blot analysis of beta Catenin (non-phospho(active) S37/T41) using ab246504 at 1:1000 (0.541 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

    Lane 1: beta Catenin non-phospho-Ser33/Ser37/Thr41 peptide (aa 29-44)
    Lane 2: beta Catenin non-phospho-Ser33/Ser37/Thr41 peptide (aa 31-47)
    Lane 3: beta Catenin phospho-Ser33 non-phospho-Ser37/Thr41 peptide (aa 29-44)
    Lane 4: beta Catenin phospho-Ser37 non-phospho-Ser33/Thr41peptide (aa 29-44)
    Lane 5: beta Catenin phospho-Thr41 non-phospho-Ser33/Ser37 peptide (aa 29-47)
    Lane 6: beta Catenin phospho-Ser33/Ser37 non-phospho-Thr41 peptide (aa 29-47)
    Lane 7: beta Catenin phospho-Ser33/Thr41 non-phospho-Ser37 peptide (aa 29-47)
    Lane 8: beta Catenin phospho-Ser37/Thr41non-phospho-Ser33 peptide (aa 29-47)
    Lane 9: beta Catenin phospho-Ser33/Ser37/Thr41peptide (aa 29-47)

    Exposure time: 81 seconds

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

  • Immunoprecipitation - Anti-beta Catenin antibody [EPR23969-131] - BSA and Azide free (ab278503)
    Immunoprecipitation - Anti-beta Catenin antibody [EPR23969-131] - BSA and Azide free (ab278503)

    This data was developed using ab246504, the same antibody clone in a different buffer formulation.

    beta Catenin (non-phospho(active) S37/T41) was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab246504 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246504 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab246504 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246504 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds

     

     

  • Anti-beta Catenin antibody [EPR23969-131] - BSA and Azide free (ab278503)
    Anti-beta Catenin antibody [EPR23969-131] - BSA and Azide free (ab278503)

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