Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free
See all Metabotropic Glutamate Receptor 3/MGLUR3 primary antibodies
Description

Rabbit monoclonal [EPR24635-155] to Metabotropic Glutamate Receptor 3/MGLUR3 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Fr, IHC-Pmore details
Unsuitable for: Flow Cyt or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse brain, Rat brain, Human brain, HEK-293T transfected with Metabotropic glutamate receptor 3 expression vector containing a myc-His-tag® whole lysates. IHC-P: Mouse cerebrum, Mouse cerebellum, Human cerebrum and Rat cerebrum tissues. IHC-Fr: Mouse hippocampus, Rat hippocampus tissues.
General notes
ab281942 is the carrier-free version of ab281922. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab281942 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

Constituent: 100% PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24635-155
Isotype

IgG

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

All lanes : Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] (ab281922) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate at 20 µg
Lane 2 : Mouse liver tissue lysate at 40 µg
Lane 3 : Rat brain tissue lysate at 20 µg
Lane 4 : Rat liver tissue lysate at 40 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 99 kDa
Observed band size: 100,250 kDa why is the actual band size different from the predicted?

This data was developed using 281922, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Negative control: liver (PMID: 9001707).

The molecular weight observed is consistent with what has been described in the literature (PMID: 27130562).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time: 3.25 seconds.
Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] (ab281922) at 1/1000 dilution + Human brain tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 99 kDa
Observed band size: 100,250 kDa why is the actual band size different from the predicted?

This data was developed using 281922, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature (PMID: 27130562).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time: 10 seconds.
Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

All lanes : Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] (ab281922) at 1/1000 dilution

Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with Metabotropic glutamate receptor 3 expression vector containing a myc-His-tag®, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 99 kDa
Observed band size: 100,250 kDa why is the actual band size different from the predicted?

This data was developed using 281922, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature (PMID: 27130562).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time: 1 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebellum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cerebrum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101). Negative control: No staining on mouse liver. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (3.42 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Cytoplasmic staining on mouse hippocampus is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (3.42 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Cytoplasmic staining on rat hippocampus is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (6.84 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Negative control: No staining on mouse liver (PMID: 9001707) is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

This data was developed using ab281922, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (6.84 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Negative control: No staining on rat liver (PMID: 9001707) is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.
Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)