Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24635-155] to Metabotropic Glutamate Receptor 3/MGLUR3 - BSA and Azide free
  • Suitable for: WB, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free
    See all Metabotropic Glutamate Receptor 3/MGLUR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR24635-155] to Metabotropic Glutamate Receptor 3/MGLUR3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain, Rat brain, Human brain, HEK-293T transfected with Metabotropic glutamate receptor 3 expression vector containing a myc-His-tag® whole lysates. IHC-P: Mouse cerebrum, Mouse cerebellum, Human cerebrum and Rat cerebrum tissues. IHC-Fr: Mouse hippocampus, Rat hippocampus tissues.

  • General notes

    ab281942 is the carrier-free version of ab281922. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281942 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24635-155

  • Isotype

    IgG

Images

  • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    All lanes : Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] (ab281922) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate at 20 µg
    Lane 2 : Mouse liver tissue lysate at 40 µg
    Lane 3 : Rat brain tissue lysate at 20 µg
    Lane 4 : Rat liver tissue lysate at 40 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 99 kDa
    Observed band size: 100,250 kDa why is the actual band size different from the predicted?



    This data was developed using 281922, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: liver (PMID: 9001707).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 27130562).

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: 3.25 seconds.

     

  • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] (ab281922) at 1/1000 dilution + Human brain tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 99 kDa
    Observed band size: 100,250 kDa why is the actual band size different from the predicted?



    This data was developed using 281922, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 27130562).

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: 10 seconds.

     

  • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    All lanes : Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] (ab281922) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with Metabotropic glutamate receptor 3 expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 99 kDa
    Observed band size: 100,250 kDa why is the actual band size different from the predicted?



    This data was developed using 281922, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 27130562).

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: 1 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

    This data was developed using ab281922, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

    This data was developed using ab281922, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebellum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

    This data was developed using ab281922, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cerebrum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

    This data was developed using ab281922, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

    This data was developed using ab281922, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101). Negative control: No staining on mouse liver. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

    This data was developed using ab281922, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (3.42 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Cytoplasmic staining on mouse hippocampus is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

    This data was developed using ab281922, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (3.42 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Cytoplasmic staining on rat hippocampus is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

    This data was developed using ab281922, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (6.84 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: No staining on mouse liver (PMID: 9001707) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

    This data was developed using ab281922, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (6.84 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: No staining on rat liver (PMID: 9001707) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

  • Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)
    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-155] - BSA and Azide free (ab281942)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.