Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [2A6] to ALDH1L1
Suitable for: IHC-P, WB, Flow Cyt
Reacts with: Rat, Dog, Human, African green monkey
Isotype: IgG1

Product name

Anti-ALDH1L1 antibody [2A6]
See all ALDH1L1 primary antibodies
Description

Mouse monoclonal [2A6] to ALDH1L1
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human ALDH1L1.
Database link: O75891
Positive control
- WB: HepG2, HeLa, HT-29, A549, COS-7, MDCK, PC-12 and pCMV6-ENTRY ALDH1L1-transfected HEK-293T whole cell lysate. IHC-P: Human kidney and liver tissue. Flow cyt: Jurkat cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

2A6
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] (ab231018)

Formalin-fixed, paraffin-embedded human kidney tissue stained for ALDH1L1 using ab231018 at 10 μg/ml in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [2A6] (ab231018)

Formalin-fixed, paraffin-embedded human liver tissue stained for ALDH1L1 using ab231018 at 10 μg/ml in immunohistochemical analysis.
Western blot - Anti-ALDH1L1 antibody [2A6] (ab231018)

All lanes : Anti-ALDH1L1 antibody [2A6] (ab231018) at 1/500 dilution

Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : pCMV6-ENTRY ALDH1L1-transfected HEK-293T whole cell lysate

Lysates/proteins at 5 µg per lane.

Developed using the ECL technique.
Western blot - Anti-ALDH1L1 antibody [2A6] (ab231018)

All lanes : Anti-ALDH1L1 antibody [2A6] (ab231018) at 1/500 dilution

Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : MDCK (canine kidney cell line) whole cell lysate
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 35 µg per lane.

Developed using the ECL technique.
Flow Cytometry - Anti-ALDH1L1 antibody [2A6] (ab231018)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling ALDH1L1 with ab231018 at 1/100 dilution (red) compared with a non-specific negative control antibody (blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com