Anti-Rubicon/Baron antibody - C-terminal (ab229499)
Key features and details
- Rabbit polyclonal to Rubicon/Baron - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rubicon/Baron antibody - C-terminal
See all Rubicon/Baron primary antibodies -
Description
Rabbit polyclonal to Rubicon/Baron - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment within Human Rubicon/Baron (C terminal). The exact sequence is proprietary.
Database link: Q92622 -
Positive control
- WB: NTERA-2 cl.D1 [N2/D1] and Rubicon/Baron-transfected HEK-293T whole cell extracts. IP: Rubicon/Baron-transfected HEK-293T whole cell extract.
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General notes
This product was previously labelled as Rubicon
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Rubicon/Baron antibody - C-terminal (ab229499)Anti-Rubicon/Baron antibody - C-terminal (ab229499) at 1/1000 dilution + NTERA-2 cl.D1 [N2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell extract at 30 µg
Predicted band size: 109 kDa5% SDS-PAGE gel.
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Western blot - Anti-Rubicon/Baron antibody - C-terminal (ab229499)All lanes : Anti-Rubicon/Baron antibody - C-terminal (ab229499) at 1/20000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Rubicon/Baron, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 109 kDa5% SDS-PAGE gel.
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Immunoprecipitation - Anti-Rubicon/Baron antibody - C-terminal (ab229499)Rubicon/Baron was immunoprecipitated from Rubicon/Baron-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 4 µg of ab229499. Western blot was performed from the immunoprecipitate using ab229499 at 1/1000 dilution. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Rubicon/Baron transfected HEK-293T whole cell extract 30 µg (Input).
Lane 2: 4 µg preimmune Rabbit IgG instead of ab229499 in Rubicon transfected HEK-293T whole cell extract.
Lane 3: ab229499 IP in Rubicon transfected HEK-293T whole cell extract.
