Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ALDH1L1 antibody [EPR12743(B)] - BSA and Azide free (ab233016)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12743(B)] to ALDH1L1 - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ALDH1L1 antibody [EPR12743(B)] - BSA and Azide free
    See all ALDH1L1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12743(B)] to ALDH1L1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney tissue. IHC-Fr: Mouse and rat liver tissue.

  • General notes

    Ab233016 is the carrier-free version of ab177463. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab233016 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - BSA and Azide free (ab233016)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - BSA and Azide free (ab233016)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH1L1 with ab177463 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177463).

  • Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - BSA and Azide free (ab233016)
    Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - BSA and Azide free (ab233016)
    Immunohistochemistry (Frozen sections) analysis of mouse liver tissue labeling ALDH1L1 with ab177463 at 1/100 dilution. Tissue was fixed with 4% PFA and permeabilised with 0.2% Triton X-100. ab150077, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. DAPI (blue) was used as nucleare counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Cytoplasmic staining on mouse liver.
     
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177463).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - BSA and Azide free (ab233016)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - BSA and Azide free (ab233016)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALDH1L1 with ab177463 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177463).

  • Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - BSA and Azide free (ab233016)
    Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - BSA and Azide free (ab233016)
    Immunohistochemistry (Frozen sections) analysis of rat liver tissue labeling ALDH1L1 with ab177463 at 1/100 dilution. Tissue was fixed with 4% PFA and permeabilised with 0.2% Triton X-100. ab150077, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. DAPI (blue) was used as nucleare counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Cytoplasmic staining on rat liver.
     
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177463).
  • Anti-ALDH1L1 antibody [EPR12743(B)] - BSA and Azide free (ab233016)
    Anti-ALDH1L1 antibody [EPR12743(B)] - BSA and Azide free (ab233016)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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