Anti-Thrombospondin antibody - N-terminal (ab223472)
Key features and details
- Goat polyclonal to Thrombospondin - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Thrombospondin antibody - N-terminal
See all Thrombospondin primary antibodies -
Description
Goat polyclonal to Thrombospondin - N-terminal -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Synthetic peptide corresponding to Human Thrombospondin aa 1-100 (N terminal) (Cysteine residue). (NP_003237.2).
Database link: P07996 -
Positive control
- WB: HEK-293 lysate overexpressing Human Thrombospondin with C-terminal MYC tag.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab223472 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Thrombospondin antibody - N-terminal (ab223472)Anti-Thrombospondin antibody - N-terminal (ab223472) at 1 µg/ml + Mouse kidney lysate at 35 µg
Predicted band size: 129 kDaDetected by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody - N-terminal (ab223472)Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (4µg/ml), showing cytoplasmic/Plasma Membrane staining. The nuclear -
Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody - N-terminal (ab223472)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using ab223472. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (4µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody - N-terminal (ab223472)Ab223472 (5µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. -
Western blot - Anti-Thrombospondin antibody - N-terminal (ab223472)Lanes 1-2 : Anti-Thrombospondin antibody - N-terminal (ab223472) at 0.1 µg/ml
Lane 3 : anti-MYC Tag at 1/1000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) lysate overexpressing Human Thrombospondin with C-terminal MYC (in RIPA buffer)
Lane 2 : Mock transfected HEK-293 lysate (in RIPA buffer)
Lane 3 : HEK-293 lysate overexpressing Human Thrombospondin with C-terminal MYC (in RIPA buffer)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 129 kDaPrimary incubations were for 1 hour.
In transfected HEK-293 transiently expressing Human Thrombospondin a band of approx. 200kDa plus a breakdown product are observed. These bands are not observed in the non-transfected HEK-293.
