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Anti-Integrin alpha V+beta 5 antibody [P1F6] (ab177004)

Key features and details

  • Mouse monoclonal [P1F6] to Integrin alpha V+beta 5
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Integrin alpha V+beta 5 antibody [P1F6]
    See all Integrin alpha V+beta 5 primary antibodies

  • Description

    Mouse monoclonal [P1F6] to Integrin alpha V+beta 5

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus corresponding to Human Integrin alpha V+beta 5. Small cell lung carcinoma cells (UCLA P3).

  • Positive control

    • This antibody gave a positive signal in IF and Flow Cytometry in A549 cells.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V+beta 5 antibody [P1F6] (ab177004)
    Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V+beta 5 antibody [P1F6] (ab177004)

    ab177004 stained in A549 cells. Cells were fixed with 4% paraformaldehyde (10 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1 h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab177004 at 5 µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150080 (pseudo-colored red) and ab150117 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Flow Cytometry - Anti-Integrin alpha V+beta 5 antibody [P1F6] (ab177004)
    Flow Cytometry - Anti-Integrin alpha V+beta 5 antibody [P1F6] (ab177004)

    Flow Cytometry Overlay histogram showing A549 cells stained with ab177004 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab177004) (1x106 cells in 100µl at 1µg/ml) for 30 min on ice.

    The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min on ice.

    Isotype control antibody (black line) was mouse IgG1κ; (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  • Flow Cytometry - Anti-Integrin alpha V+beta 5 antibody [P1F6] (ab177004)
    Flow Cytometry - Anti-Integrin alpha V+beta 5 antibody [P1F6] (ab177004)

    Overlay histogram showing A549 cells stained with ab177004 (red line). The cells were fixed with 4% formaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab177004, 0.1μg/1x106 cells) for 30 min at 22ºC.

    The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22ºC.

    Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

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