Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Tuesday March 16, 2021

Anti-WISP1 antibody [EPR22852-267] (ab260036)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22852-267] to WISP1
Suitable for: IP, WB
Reacts with: Mouse, Rat

Product name

Anti-WISP1 antibody [EPR22852-267]
See all WISP1 primary antibodies
Description

Rabbit monoclonal [EPR22852-267] to WISP1
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

Rat

WB
Mouse

Rat

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: B16-F10, C2C12, MEF, NIH/3T3 and L6 lysates. IP: C2C12 and L6 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IP	Mouse Rat
WB	Mouse Rat

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22852-267
Isotype

IgG
Research areas

Western blot - Anti-WISP1 antibody [EPR22852-267] (ab260036)

Anti-WISP1 antibody [EPR22852-267] (ab260036) at 1/1000 dilution + L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 40 kDa
Observed band size: 30,41 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 20010302, 30723155,17383426, 12064632), the 30kDa band is the splice variant of WISP1 as described in the literature (PMID: 17383426) Exposure time: 81 seconds.
Western blot - Anti-WISP1 antibody [EPR22852-267] (ab260036)

All lanes : Anti-WISP1 antibody [EPR22852-267] (ab260036) at 1/1000 dilution

Lane 1 : B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate
Lane 2 : C2C12 (mouse myoblasts myoblast) whole cell lysate
Lane 3 : MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 40 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 20010302, 30723155,17383426, 12064632), the 30kDa band is the splice variant of WISP1 as described in the literature (PMID: 17383426) Exposure time: Lane 1:37 seconds; Lane 2:59 seconds; Lanes 3: 3 minutes; Lane 4:15 seconds.
Immunoprecipitation - Anti-WISP1 antibody [EPR22852-267] (ab260036)

WISP1 was immunoprecipitated from 0.35 mg L6 (rat skeletal muscle myoblast) whole cell lysate with ab260036 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260036 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1: L6 (rat skeletal muscle myoblast) whole cell lysate 10ug

Lane 2: ab260036 IP in L6 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260036 in L6 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 min
Immunoprecipitation - Anti-WISP1 antibody [EPR22852-267] (ab260036)

WISP1 was immunoprecipitated from 0.35 mg C2C12 (mouse myoblasts myoblast) whole cell lysate with ab260036 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260036 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1: C2C12 (mouse myoblasts myoblast) whole cell lysate 10ug

Lane 2: ab260036 IP in C2C12 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260036 in C2C12 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Anti-WISP1 antibody [EPR22852-267] (ab260036)

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