Anti-WISP1 antibody - C-terminal (ab178547)
Key features and details
- Rabbit polyclonal to WISP1 - C-terminal
- Suitable for: IHC-P, ICC, IHC-Fr, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-WISP1 antibody - C-terminal
See all WISP1 primary antibodies -
Description
Rabbit polyclonal to WISP1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human WISP1 aa 354-367 (C terminal). Differs from the related mouse and rat sequences by one amino acid.
Sequence:ADLESYPDFSEIAN
Database link: O95388 -
Positive control
- WB: Rat heart, kidney and lung tissue lysates; A549, A431 and COLO320 cell lysates. IHC-P: Rat kidney and pancreas tissue; Human placenta tissue ICC: MCF7 cells. IHC-Fr: Rat cardiac muscle tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling WISP1 with ab178547 at 1 µg/ml.
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All lanes : Anti-WISP1 antibody - C-terminal (ab178547) at 0.5 µg/ml
Lane 1 : Rat heart tissue lysate at 50 µg
Lane 2 : Rat kidney tissue lysate at 50 µg
Lane 3 : Rat lung tissue lysate at 50 µg
Lane 4 : A549 whole cell lysate at 40 µg
Lane 5 : A431 whole cell lysate at 40 µg
Lane 6 : COLO320 whole cell lysate at 40 µg
Predicted band size: 40 kDa
Observed band size: 40 kDa
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Immunohistochemical analysis of frozen rat cardiac muscle sections labeling WISP1 with ab178547 at 1μg/mL (incubated overnight at 4°C). Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling WISP1 with ab178547 at 1 µg/ml.
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling WISP1 with ab178547 at 1 µg/ml.
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Immunocytochemical analysis of MCF7 cells labeling WISP1 with ab178547 at 1 µg/ml.