Anti-WISP1 antibody [EPR22852-267] - BSA and Azide free (ab263948)
![Anti-WISP1 antibody [EPR22852-267] - BSA and Azide free (ab263948)](https://www.abcam.com/ps/products/263/ab263948/Images/ab263948-355828-antiwisp1antibodyepr22852267immunoprecipitationl6rat.jpg "Anti-WISP1 antibody [EPR22852-267] - BSA and Azide free (ab263948)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22852-267] to WISP1 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-WISP1 antibody [EPR22852-267] - BSA and Azide free
See all WISP1 primary antibodies -
Description
Rabbit monoclonal [EPR22852-267] to WISP1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Rat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: B16-F10, C2C12, MEF, NIH/3T3 and L6 lysates. IP: C2C12 and L6 cells.
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General notes
ab263948 is the carrier-free version of ab260036. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab263948 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22852-267 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-WISP1 antibody [EPR22852-267] - BSA and Azide free (ab263948)
WISP1 was immunoprecipitated from 0.35 mg L6 (rat skeletal muscle myoblast) whole cell lysate with ab260036 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260036 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: L6 (rat skeletal muscle myoblast) whole cell lysate 10ug
Lane 2: ab260036 IP in L6 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260036 in L6 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 min
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260036).
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![Immunoprecipitation - Anti-WISP1 antibody [EPR22852-267] - BSA and Azide free (ab263948)](https://www.abcam.com/ps/products/263/ab263948/Images/ab263948-355827-antiwisp1antibodyepr22852267immunoprecipitationc2c12mouse.jpg)
Immunoprecipitation - Anti-WISP1 antibody [EPR22852-267] - BSA and Azide free (ab263948)WISP1 was immunoprecipitated from 0.35 mg C2C12 (mouse myoblasts myoblast) whole cell lysate with ab260036 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260036 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: C2C12 (mouse myoblasts myoblast) whole cell lysate 10ug
Lane 2: ab260036 IP in C2C12 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260036 in C2C12 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260036).
-
![Anti-WISP1 antibody [EPR22852-267] - BSA and Azide free (ab263948)](https://www.abcam.com/ps/products/263/ab263948/Images/ab263948-3-benefits-of-recombinant-antibodies.png)
Anti-WISP1 antibody [EPR22852-267] - BSA and Azide free (ab263948)
