Caspase Family Colorimetric Substrate Kit Plus (ab102486)
Key features and details
- Assay type: Enzyme activity
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Caspase Family Colorimetric Substrate Kit Plus
See all Caspase Family kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Enzyme activity -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's Caspase Family Colorimetric Substrate Kit Plus provides ready-to-use colorimetric substrates for members of caspase family proteases. All substrates are provided in liquid ready-to-use form.
This kit is the idea tool to test which caspases are activated when apoptosis is induced in a particular cell type.
Visit our FAQs page for tips and troubleshooting. -
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 25 tests 2X Reaction Buffer 1 x 20ml Caspase 6 Substrate, Ac-VEID-PNA 1 x 125µl Caspase-1 Substrate, Ac-YVAD-pNA 1 x 125µl Caspase-2 Substrate, Ac-VDVAD-pNA 1 x 125µl Caspase-3 Substrate, Ac-DEVD-pNA 1 x 125µl Caspase-5 Substrate, Ac-WEHD-pNA 1 x 125µl Caspase-8 Substrate, Ac-IETD-pNA 1 x 125µl Caspase-9 Substrate, Ac-LEHD-pNA 1 x 125µl Cell Lysis Buffer 1 x 100ml Dilution Buffer 1 x 160ml DTT 1 x 400µl -
Research areas
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Relevance
Caspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. -
Cellular localization
Cytoplasmic -
Alternative names
- CASP
- CASP 2
- CASP1
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